BMBL Biosafety Assessment

Have you classified all biological agents by risk group (RG1/RG2/RG3/RG4)?*

Risk assessment based on pathogenicity, transmission route, availability of treatment

Are laboratory spaces assigned appropriate biosafety levels (BSL-1/2/3/4)?*

BSL must match risk group of agents handled

Do you maintain an inventory of all biological agents and their locations?*

Are Select Agents registered with CDC/APHIS if applicable?

Federal registration required for Select Agents and Toxins

Does your BSL-2+ laboratory have a sink for handwashing located near the exit?*

Required for BSL-2 and above

Are biosafety cabinets (BSC) certified annually and functioning properly?*

Class II BSC required for BSL-2 with aerosol-generating procedures

Is an autoclave available and qualified for decontamination?*

Biological indicator testing required for validation

For BSL-3, is the laboratory separated from public corridors with double-door access?

Directional airflow and dedicated HVAC required for BSL-3

Are appropriate PPE requirements defined and enforced for each biosafety level?*

Lab coats, gloves, eye protection as required by risk assessment

Do you provide respiratory protection (N95/PAPR) for aerosol-generating procedures?*

Required when BSC unavailable or for certain BSL-3 work

Are personnel trained on proper donning, doffing, and disposal of PPE?*

Is dedicated laboratory PPE prohibited from leaving the laboratory?*

Lab coats must not be worn in non-laboratory areas

Are written SOPs available for all procedures involving biohazardous materials?*

Do SOPs address spill cleanup, decontamination, and waste disposal?*

Specific procedures for liquid spills, sharps, and contaminated equipment

Are aerosol-generating procedures identified and controlled?*

Centrifugation, vortexing, pipetting performed in BSC

Is eating, drinking, smoking, and applying cosmetics prohibited in the laboratory?*

Do you offer medical surveillance (baseline serology, vaccinations) for at-risk personnel?*

Pre-exposure vaccinations when available (HBV, rabies, etc.)

Are occupational health services available for post-exposure evaluation?*

Access to healthcare for exposures and potential infections

Do you maintain confidential medical records for exposed personnel?*

Do you have a documented biosafety incident response plan?*

Spills, exposures, releases, equipment failures

Are all laboratory incidents and exposures documented and investigated?*

Root cause analysis and corrective actions

Do you report Select Agent releases to CDC/APHIS within required timeframes?

24-hour reporting for Select Agent incidents